Total Flavonoid Content and Antioxidant Activity by Different Drying and Extraction Methods of Clinacanthus nutans Leaves

Mohd Hafiz Abd Majid, and Yvonne Melse Laurence, and Mohammad Shaheen Khan, and Mohd Sani Sarjadi, (2018) Total Flavonoid Content and Antioxidant Activity by Different Drying and Extraction Methods of Clinacanthus nutans Leaves. BORNEO SCIENCE, 39 (2). pp. 72-85.

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Abstract

This report presents a study on the total flavonoid content and antioxidant activity of Clinacanthus nutans leaves from different drying and extraction methods. The C. nutans leaves were subjected through three different drying methods: oven (40°C, 60°C and 80°C), vacuum oven and air drying. Afterwards, extraction on the dried leaves was performed using three different extraction techniques: soxhlet, maceration and ultrasoundassisted solvent extraction. Finally, total flavonoid and antioxidantactivity was determined spectrophotometrically by aluminium chloride colorimetric assay and 2-Diphenyl-1-picryl hydrazyl (DPPH) method respectively. The results showed that the choice of drying and extraction methods had a significant influence on total flavonoid and antioxidant content of C. nutans extract. The combination of drying C. nutans leaves using laboratory oven at 60°C and soxhlet extraction obtained the highest amount of total flavonoid and antioxidant content at 24.53 ± 0.95 mg RU/g and 89.73 ± 4.39mg TE/ g respectively. The highest antioxidant activity for C. nutansfromair-dried samples and vacuum oven-dried samples were obtained through maceration extraction at 85.46 mg TE/ g and 83.96 mg TE/ g respectively.

Item Type: Article
Uncontrolled Keywords: Clinacanthus nutans, Drying, Extraction method, Flavonoid, Antioxidant
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 14 Nov 2019 06:01
Last Modified: 14 Nov 2019 06:01
URI: http://eprints.ums.edu.my/id/eprint/24092

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