An optimal blend of single auto displayed cellulases for cellulose saccharification – a proof of concept

Eugene Marfo Obeng, and Clarence M. Ongkudon, and Cahyo Budiman, and Ruth Maas, and Joachim Jose, (2018) An optimal blend of single auto displayed cellulases for cellulose saccharification – a proof of concept. Journal of Chemical Technology & Biotechnology. pp. 1-10.

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Abstract

BACKGOUND: To date, it has been challenging to determine the optimal proportions of the individual cellulases in crude extracts and multienzyme systems to promote enzyme synergism. The application of single surface-display of enzymes may obviate such challenges. This article presents a proof of concept of the optimal mixing of single surface-displayed cellulases for cellulose hydrolysis using Simplex lattice mixture design. RESULTS: The recently discovered maximized autotransporter-mediated expression system was used to express the three cellulases. The biochemical screening analysis revealed that the enzymes have a broad range of functional pH (4–9) and temperature (30–100 ∘C) characteristics. The evaluated optima were pH 6 and 60 ∘C. Through the statistical design of experiment, a blend ratio of 1: 1.6: 1.4 of endoglucanase: exoglucanase:

Item Type: Article
Uncontrolled Keywords: cellulases, synergism, autodisplay technology, bacterial surface display, enzyme blend, mixture design
Subjects: T Technology > T Technology (General)
T Technology > TP Chemical technology
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 26 Nov 2019 06:15
Last Modified: 26 Nov 2019 06:15
URI: http://eprints.ums.edu.my/id/eprint/24196

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