Method for the separation of mitochondria and apicoplast from the malaria parasite Plasmodium falciparum

Masayuki Hata, and Shigeharu Sato, and Kiyoshi Kita, (2019) Method for the separation of mitochondria and apicoplast from the malaria parasite Plasmodium falciparum. Parasitology International, 69. pp. 99-102.

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Abstract

The growth and the survival of the human malaria parasite Plasmodium falciparum are critically dependent on the functions of the two organelles - the mitochondrion and the apicoplast. However, these two organelles have been known to be difficult to separate from each other when they are released from Plasmodium cell. We have been searching for the conditions with which separation of the mitochondrion and the apicoplast is achieved. In this study, we investigated how the two organelle's separation is affected when the pressure of the nitrogen gas to disrupt the Plasmodium cells by nitrogen cavitation method is lowered from the pressure regularly applied (1200 psi). The parasite cell was sufficiently disrupted even when nitrogen cavitation was carried out at 300 psi. The obtained mitochondrial sample was much less contaminated by DNA compared with the sample prepared using the gas at the regular pressure. After the fractionation by Percoll density gradient, the mitochondrion and the apicoplast from the 300 psi cell lysate exhibited different separation profiles. This is the first experimental evidence that indicates the mitochondrion and the apicoplast of P. falciparumare separable from each other.

Item Type: Article
Uncontrolled Keywords: Apicoplast, Cell fractionation, Malaria, Mitochondrion, Nitrogen cavitation, Plasmodium falciparum
Subjects: R Medicine > R Medicine (General)
R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: FACULTY > Faculty of Medicine and Health Sciences
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 04 Dec 2019 01:08
Last Modified: 04 Dec 2019 01:08
URI: http://eprints.ums.edu.my/id/eprint/24266

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