Qualitative evaluation of slaughterhouse Goat Ovaries by histochemical study

Siti Mariam Azalan, (2017) Qualitative evaluation of slaughterhouse Goat Ovaries by histochemical study. Universiti Malaysia Sabah. (Unpublished)

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Abstract

A histological study of mature goat ovaries was carried out at the Animal Anatomy and Physiology Laboratory, Faculty of Sustainable Agriculture, Universiti Malaysia Sabah (UMS) Sandakan Campus from May 2016 until October 2016. The objectives of this study are to identify the quality of follicles in goat ovaries and to find out the relationship between the quality of follicles and ovarian state. The animals used in this study were 2 adult female goats (doe) with unknown reproductive background that have been slaughtered at Hafiz Farm slaughterhouse in Lahad Datu. The ovaries were categorized as right, left, corpus luteum (CL) present (CL+) and absent (CL-). The categorized ovaries were evaluated physically and histologically by using light microscope. The mean weight (g), length (cm), and width (cm) of right ovary was distinctly higher [(0.67±0.02)g, (1.16±0.03)cm, (0.75±0.15)cm respectively] than the left ovary. On the other hand, the ovary with CL have higher weight and width [(0.69)g, (0.9)cm respectively], while the mean length of ovary without CL were found higher [(1.15±0.02)cm]. The follicles were categorized into few stages which are the primordial, primary, secondary, early antral, antral and preovulatory follicles by histological means. From the classification, higher number of primordial, primary and secondary follicles [(675.5±619.50), (145.0±81.0) and (53.5±38.50) respectively] was found in right ovaries. However, the number of preovulatory follicles counted was found higher in left ovary (7.5±5.5) compared to right. From other point of view, total number of primordial, primary and secondary follicles observed higher in ovary with CL (1295, 226 and 92 respectively) compared to ovary without CL except the preovulatory follicles which is higher (5.33±3.84) than with CL (3). From qualitative evaluation, a high number of abnormal follicles were found in the right ovaries (58.5±52.5) while higher number of normal follicles was found in left ovaries with (60.0±4.0). In respect of CL present or absent, higher number of normal and abnormal follicles were found in ovary with CL [(51 and 111) respectively] compared to ovaries without CL [(43.0±17.16) and (28.67±11.35) respectively]. The present findings revealed that right and left ovaries both have a great potentiality to provide good number of oocytes for in vitro studies. Considering with the effects of CL on ovaries and low number of sample, highest number and normal grade follicle would be collected from ovaries without CL. Moreover, this result can be used to collect quality follicles for ooc.yte aspiration for IVEP thus creates a great opportunity for conducting further research on goat embryo production especially here in Sabah, Malaysia.

Item Type: Academic Exercise
Uncontrolled Keywords: Animal Anatomy , goat ovaries , Sabah
Subjects: S Agriculture > S Agriculture (General)
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: Noraini
Date Deposited: 03 Jan 2020 00:08
Last Modified: 03 Jan 2020 00:08
URI: http://eprints.ums.edu.my/id/eprint/24534

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