Phytochemical analysis of Etlingera brevilabrum (Valeton) R. M. Sm.

Chan, Jinn Yang (2018) Phytochemical analysis of Etlingera brevilabrum (Valeton) R. M. Sm. Universiti Malaysia Sabah. (Unpublished)

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Abstract

In this study, the effects of red pigmentation coverage on leaf surface of Etlingera breveilabrum (Valeton) R. M. Sm. and extraction time on total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AOA) and antimicrobial activity (AMA) were investigated. The leaves of E. brevilabrum were divided into three categories based on the red pigmentation coverage on the leaf surface; Ll (70 -100%), L2 (35 -69%) and L3 (0 -34%). The leaves samples were macerated with methanol solvent and extracted at two different extraction time, El: 72 hours or E2: 1 hour. The TPC and TFC were estimated using spectrophotometric method. The AOA of extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The AMA of extracts was evaluated using Kirby-Bauer test. The results showed that, there was no interaction between the two factors of red pigmentation coverage on the leaf surface and the extraction time of E. brevilabrum. However, the factor of red pigmentation coverage did influence the extraction yield and TFC. The highest extraction yield was observed on L3 (41.33 ± 9.18 %) followed by L2 (34.50 ± 8.76 %) and L1 (33.33 ± 7.89%). For TFC, Ll produced higher TFC (2.48 ± 0.24 mg QE/g) compared to L2 (2.40 ± 0.14 mg QE/g) and L3 (2.27 ± 0.08 mg QE/g). Meanwhile, the factor of extraction time was found to influence all parameters tested. Leaf sample extracted for 72 hours gave higher extraction yield which is 44.33 ± 6.24 %, however, sample that was extracted for 1 hour showed better result for TPC, TFC, and AOA with 47 .62 ± 5.14 mg GAE/g, 2.50 ± 0.16 mg QE/g and ICso value of 430.43 ± 55.71 µg/ml respectively. A strong correlation was observed between the TPC and AOA, whereas the TFC showed weak correlation with the AOA. For the antimicrobial activity, it was found that only leaves sample extracted for 1 hour showed inhibition against 8aCJ7lus cereus. As for recommendations, further research on the effect of extraction time on E. brevilabrum leaves can be carried out in order to determine the optimal extraction time of this plant. In addition, further research on the antimicrobial activity of E. brevilabrum can be carried out to determine the minimal inhibitory concentration required to inhibit the growth of bacteria.

Item Type: Academic Exercise
Uncontrolled Keywords: Red pigmentation coverage , Etlingera breveilabrum
Subjects: S Agriculture > S Agriculture (General)
Divisions: FACULTY > Faculty of Sustainable Agriculture
Depositing User: Noraini
Date Deposited: 29 Jan 2020 02:23
Last Modified: 29 Jan 2020 02:23
URI: http://eprints.ums.edu.my/id/eprint/24692

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