Effect of extraction conditions of Carica papaya leaves aqueous extracts and its resulting infusion with “kelulut” honey to its antioxidant activity

Monjia Belleza Cosmas Mojulat, and Noumie Surugau, (2018) Effect of extraction conditions of Carica papaya leaves aqueous extracts and its resulting infusion with “kelulut” honey to its antioxidant activity. ASM Science Journal, 11 (2). pp. 75-86.

[img]
Preview
Text
Effect of extraction conditions of Carica papaya leaves aqueous extracts and its resulting infusion with “kelulut” honey to its antioxidant activity.pdf

Download (135kB) | Preview
[img]
Preview
Text
Effect of extraction conditions of Carica papaya leaves aqueous extracts and its resulting infusion with “kelulut” honey to its antioxidant activity1.pdf

Download (357kB) | Preview

Abstract

Carica papaya is a tropical plant belonging to the Caricaceae family. Rich in phytochemical content, its leaves especially are renowned for having many health benefits, namely anticancer properties and as supplement in treating dengue disease. This study thus aimed to optimise aqueous extraction and to determine the effect of honey infusion on the leaves extract which was analysed using TPC, TFC, FRAP and DPPH assays respectively. The optimal extraction conditions for aqueous extraction were determined to be at 70˚C for 20 minutes where its TPC was 9.97±0.47 mg GAE/mL (p<0.05), TFC was totaled at 2.63±0.52 mg QUE/mL (p<0.05) while its FRAP assay was amounted at 16.84±1.10 mg TE/mL. Radical scavenging values using DPPH assay was recorded to be at 87.53% with its IC50 at 492.54±2.45 mg TE/mL (p<0.05). Study on the infusion of “kelulut” honey from Trigona species with the leaves extract provides evidence that not only does it improve the taste of the bitter papaya extract; the positive synergy also increases the overall antioxidant activity. Antioxidant values are found to increase in accordance with increasing honey dosage (max 4 tbsp.), where its TPC was valued at 21.66±0.54 mg GAE/mL (p<0.05), FRAP test at 24.02±0.87 mg TE/mL (p<0.05), radical scavenging activity at 98.2% and lower IC50 value at 408.02± 5.0 mg TE/mL. Future study can therefore be done to improve and perhaps release the product commercially.

Item Type: Article
Uncontrolled Keywords: Total Phenolic Count (TPC), Total Flavonoid Count (TFC), Ferric Reducing Antioxidant Power (FRAP), (2,2-diphenyl-1-picryl-hydrazyl-hydrate) DPPH, Carica papaya leaves
Subjects: S Agriculture > SB Plant culture
S Agriculture > SF Animal culture
Divisions: FACULTY > Faculty of Science and Natural Resources
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 09 Feb 2020 23:24
Last Modified: 19 Jun 2020 04:57
URI: http://eprints.ums.edu.my/id/eprint/24915

Actions (login required)

View Item View Item