Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides

Mailin Misson, and Bo Jin, and Sheng Dai, and Hu Zhang, (2020) Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides. Catalysts, 10 (81). pp. 1-12.

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Abstract

Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at are sidence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis.

Item Type: Article
Uncontrolled Keywords: β-galactosidase, polymer nanofibers, nanobiocatalyst, interfacial characterization, interfacial reaction, conformational change
Subjects: Q Science > Q Science (General)
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 20 Feb 2020 03:11
Last Modified: 17 Apr 2020 15:22
URI: http://eprints.ums.edu.my/id/eprint/24984

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