Candidate mutants of Solanum Lycopersicum developed using Ethyl Methane Sulfonate

Aswaneeza Khairul Aswandy, (2019) Candidate mutants of Solanum Lycopersicum developed using Ethyl Methane Sulfonate. Masters thesis, Universiti Malaysia Sabah.

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Abstract

Solanum /ycopersicum belongs to the genus of Solanum with a small genome size of 950Mb of DNA in 91 scaffold aligned to 12 tomato chromosome. Solanum /ycopersicum which has been known to be originated from Andean Region and widely used as a research material has contributed towards the vast amount of tomato genetic information such as the discovery of 34,727 protein coding gene and a mass of accumulated information of tomato functional genomics in various databases. However, the functional genomics of plant genes has not yet been fully correlated to its specific function which only 18,320 genes were specified. Despite of having a high economical value with other significant attribution and benefits, its fullness can't be achieved if vital gene function has not been known yet. The present study was performed to correlate the scrutinized targeted plant genes to its function in the basis of S. /ycopersicum mutagenesis via chemical mutagen ethyl methanesulfonate (EMS). Mutant tomato plants were developed up to its second generation mutant line which its genomic DNA were sequenced with the use of nine primers for the study of functional genomics of the specific loci in the tomato genome. All 99 samples were sequenced and screened for the changes in genotypic aspect through TILLING and reference comparison to its control untreated DNA sequence via bioinformatic software. Based on the analyzed results a total of five genes were detected to be mutated through random mutation induced in seeds by ethyl methanesulfonate treatment. The variations in 12 main classes and 24 sub­classes of tomato phenotypes, Brix sugar index, chlorophyll level and tomato height that were observed and recorded from germination stage up to senescence stage of tomato plants were in line with the polymorphism screened in the sequence of the mutated genes. Sugar index were also relatively low in mutant fruits with a mean value of 3.8 %Brix in comparison to control tomato fruits of 6.2 %Brix, indicating HXK gene might take part in the Carbon catabolite repression (CCR). Mutagenic effect of EMS were notably in the variation of mutant and control plant heights, mutant plant were stunned with the shortest to be recorded with a height of 10 cm of its main stem. Chlorophyll levels were relatively lower in leaves of mutant plants. EXP gene was screened to be mutated and caused the growth of shorter stem and nodes of tomato plants as it regulates the loosening of plant cell wall. Ethyl Methanesulfonate has proved its reliability in plant mutagenesis through the screening of newly emerging technology of TILLING for the study of plant functional genomics and further intensive research can be applied on tomato whole genome. This research of tomato as a plant model could contribute in the agriculture sector that emphasized on Solanum lycopersicum and mutagenesis via Ethyl methanesulfonate.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Solanum , chromosome , plant genes , tomato
Subjects: S Agriculture > SB Plant culture
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Noraini
Date Deposited: 11 Mar 2020 06:09
Last Modified: 11 Mar 2020 06:09
URI: http://eprints.ums.edu.my/id/eprint/25180

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