Circulating Dengue Serotypes in Sandakan and Kudat districts of Sabah: during 2016 to 2017

Timothy Jr Gintarong, (2019) Circulating Dengue Serotypes in Sandakan and Kudat districts of Sabah: during 2016 to 2017. Masters thesis, Universiti Malaysia Sabah.

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Abstract

Dengue is a mosquito-borne viral infection caused by Dengue virus (DENV). DENV is a single-stranded positive-sense RNA virus belonging to the genus Flavivirus of the family Flaviviridae with 4 serotypes (DENV-1, DENV-2, DENV-3 and DENV-4). Subsequent infection with different serotypes causes severe dengue. A total of 200 dengue rapid test positive patients' sera were collected from Hospital Duchess of Kent Sandakan and Hospital Kudat, Sabah from June 2016 to December 2017. Dengue serotyping was done at the Department of Pathobiology and Medical Diagnostics at the Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah. The OneStep Reverse Transcriptase PCR and Nested PCR were performed using C-prM amplimers. Out of 200 serum samples tested PCR positive were 128. There are 62 patients were infected with single DENV serotype and the other 66 were co-infected with more than one serotype. All dengue serotype 1 to 4 were found circulating in Sandakan and Kudat. DENV-1 was the predominant serotype and DENV-2 was the least common serotype found in both study sites. The commonest dengue infected age groups were 21 to 30 in Sandakan, and 11 to 20 years old in Kudat. Males were affected more than females in both districts with ratio of 1.2: 1. Phylogenetic analysis revealed that DENV- 1, DENV-3 and DENV-4 sequences from both Sandakan and Kudat are genetically related to sequences from Asia and Southeast Asia. The DENV sequences analysis showed DENV-1 sequences are dustered to genotype IV, DENV-3 to genotype I, and DENV-4 to genotype II. DENV-2 sequences were less than 200 nucleotides, so the phylogenetic cluster was not clearly understood.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Dengue , Dengue virus (DENV) , Flaviviridae , Sabah
Subjects: R Medicine > RC Internal medicine
Divisions: FACULTY > Faculty of Medicine and Health Sciences
Depositing User: Noraini
Date Deposited: 12 Mar 2020 08:37
Last Modified: 12 Mar 2020 08:37
URI: http://eprints.ums.edu.my/id/eprint/25221

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