Immunohistochemical, histological and ultrastructural evaluation of protection provided by cholera vaccine against V. cholerae O139

Chandrika Murugaiah, and Nik Zuraina Nik Mohd Noor, and Hassanain Al-Talib, and Shyamoli Mustafa, and Manickam Ravichandran, and Lalitha Pattabhiraman, (2020) Immunohistochemical, histological and ultrastructural evaluation of protection provided by cholera vaccine against V. cholerae O139. Microbial Pathogenesis, 140.

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Abstract

In our previous study, complete protection was observed in rabbit immunized with 1 × 1010 CFU of live attenuated VCUSM21P vaccine against challenge with 1 × 109 CFU Vibrio cholerae O139. In the present study, we investigated whether the vaccines can effectively protect immunized animals from any pathologic changes using histological, immunohistochemical and ultrastructural techniques. Severe pathology is evident in wild type injected ileum in non-immunized, showing extensive villous destruction, edema, necrosis and inflammation with infiltration of large numbers of inflammatory cells, extensive damage to the villi and microvilli with pore formation. Histology of ileum injected with wild type in immunized rabbit shows no significant pathological changes except for a few inflammatory cells in lamina propria with mild edema in mucosa and submucosa. immunohistochemical staining revealed O139 antigens of wild type are seen in the lamina propria of edematous villi, muscularis mucosa and submucosa with weak presence in the muscle coat in non-immunized rabbit after challenged with wild type in non-immunized rabbits, but in immunized rabbit localisation of the O139 LPS antigen is seen at the tips of the intact villi, within lamina propria and muscularis mucosa only. These observations suggest that the vaccine can effectively protect animals from any pathologic changes and eliminate V. cholerae O139 from the immunized animals.

Item Type: Article
Uncontrolled Keywords: cholera vaccine against, Immunohistochemical, cholera
Subjects: R Medicine > R Medicine (General)
R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: FACULTY > Faculty of Medicine and Health Sciences
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 13 Aug 2020 23:59
Last Modified: 13 Aug 2020 23:59
URI: http://eprints.ums.edu.my/id/eprint/25803

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