In vitro germination and plantlet establishment of Labisia pumila (Bl.) F. Vill.

Hartinie Marbawi, and Jualang @ Azlan Abdullah Gansau, (2007) In vitro germination and plantlet establishment of Labisia pumila (Bl.) F. Vill. Scientia Horticulturae, 115 (1). pp. 91-97. ISSN 0304-4238

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Official URL: http://dx.doi.org/10.1016/j.scienta.2007.07.011

Abstract

In vitro seeds germination and plantlet establishment of Labisia pumila were studied in this report. The seeds obtained from the mature fruits of L. pumila were sterilized and cultured on Murashige and Skoog (MS) solid media supplemented with 1-3 μM of 6-benzylaminopurine (BAP) and 3% (w/v) sucrose. The presence of BAP in the medium significantly affects seeds germination. High percentage of seeds germination (up to 90%) was successfully achieved after 2 weeks of culture on medium supplemented with 2 μM BAP. Up to 70% of explants produced shoots through direct regeneration from newly emerged epicotyls after 5 weeks of culture. The average of 8.1 ± 1.0 shoots per explant obtained on media treated with 2 μM BAP. Seedlings were further transferred to growth media fortified with different types of cytokinin. Result observed after 12 weeks showed that medium supplemented with 1 μM zeatin (ZEA) promote the highest growth with an average of 2.9 ± 1.0 cm shoot length and 7.7 ± 3.2 leaves per explant after 12 weeks. In addition, medium added with 2 μM BAP and supplemented with 3-4% (w/v) of sucrose promote the best growth i.e., 3.0 ± 0.6 shoots per explant, 2.27 ± 0.2 cm length and 4.3 ± 0.5 leaves per explant. © 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:Medicinal plant, Micropropagation, Myrsinaceae, Plant growth regulators
Subjects:Q Science > QK Botany
Divisions:SCHOOL > School of Science and Technology
ID Code:2765
Deposited By:IR Admin
Deposited On:30 Jun 2011 15:03
Last Modified:17 Feb 2015 16:32

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