Polymorphic microsatellite and cryptic simple repeat sequence markers in pineapples (Ananas comosus var. comosus)

M. J. Kinsuat, and Vijay Kumar Subbiah, (2007) Polymorphic microsatellite and cryptic simple repeat sequence markers in pineapples (Ananas comosus var. comosus). Molecular Ecology Notes, 7 (6). pp. 1032-1035. ISSN 1471-8278

[img]
Preview
Text
Polymorphic_microsatellite_and_cryptic_simple_repeat_sequence_markers_in_pineapples_(Ananas_comosus_var._comosus).pdf

Download (206kB) | Preview

Abstract

A rapid method for isolating microsatellite loci in pineapples, based on the 5′-anchored polymerase chain reaction technique, revealed 137 microsatellite loci (consisting of 62 dinucleotide, 24 trinucleotide, 49 tetranucleotide and 2 hexanucleotide repeats) and 16 cryptically simple repeat sequences. We report on the characterization of 19 polymorphic microsatellite loci and one cryptic simple repeat loci in pineapples. The number of alleles per locus ranged from two to four while the observed heterozygosity ranged from 0.1705 to 1. These markers are useful as tools for detecting levels of genetic variation in pineapple varieties for germplasm management and crossbreeding purposes. © 2007 The Authors.

Item Type: Article
Uncontrolled Keywords: 5′anchored PCR, Ananas comosus, Heterozygosity, Microsatellite, Pineapple, Polymorphism
Subjects: ?? SB354-402 ??
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 20 Apr 2011 09:16
Last Modified: 16 Oct 2017 04:17
URI: http://eprints.ums.edu.my/id/eprint/2849

Actions (login required)

View Item View Item

Browse Repository
Collection
   Articles
   Book
   Speeches
   Thesis
   UMS News
Search
Quick Search

   Latest Repository

Link to other Malaysia University Institutional Repository

Malaysia University Institutional Repository