Anti-pancreatic lipase activity of Carica papaya Linn.

Khaw, Kai Yun (2015) Anti-pancreatic lipase activity of Carica papaya Linn. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Carica papaya L. contains a broad spectrum of phytochemicals, many of which show bioactivity such as anti-obesity activity. Dietary inhibitors of fats suppressing porcine pancreatic lipase (PPL) could play an important role in obesity treatment. In this study, flesh, peel and seed of C. papaya var Eksotika and Sekaki were analyzed for their total phenolic content by Folin-Ciocalteu method, antioxidant activity by 1,1-diphenyl-2- picrylhydrazyl radical scavenging (DPPH) and anti-pancreatic lipase activity by pH titration. The samples were prepared in ethanol, water and mixture (1: 1 ethanol-water) solvent. Water extracts had the highest percentage yield compared to ethanol and mixture extracts. There were a strong correlation between antioxidant activity and total phenolic content based on Pearson correlation. The seeds extracts exhibited the highest total phenolic content and antioxidant activity, followed by peel and flesh respectively. Particularly, all samples at concentration of 0.50 mg/mL exhibited potent PPL inhibitory activity, with unripe extract of selected parts having the much stronger inhibition. Total three types unripe extracts including Eksotika flesh (mixture), Eksotika seed (mixture) and Sekaki seed (ethanol) presented the maximum inhibitory activity (> 80 %), which were lower than Orlistat as positive control (88.15 %). However, PPL inhibition was not significant correlated with total phenolic content and antioxidant activity respectively. The results demonstrated whole C. papaya could effectively inhibit the key enzymes related to obesity, especially unripe Eksotika variety had the stronger inhibition against PPL in ethanol and mixture solvent. Unripe papaya can be further applied for obesity treatment

Item Type: Academic Exercise
Uncontrolled Keywords: papaya, phenolic content, Folin-Ciocalteu method, antioxidant activity, pH titration, anti-pancreatic lipase
Subjects: T Technology > TX Home economics > Nutrition. Foods and food supply
Depositing User: Munira
Date Deposited: 28 Dec 2017 22:26
Last Modified: 28 Dec 2017 22:26
URI: http://eprints.ums.edu.my/id/eprint/33

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