Detaction of genetic in selected Sabah Park’s germplasm collection of slipper orchids (Paphiopedilum rothschildianum) using RAPD markers

Wong, Kian Yong (2004) Detaction of genetic in selected Sabah Park’s germplasm collection of slipper orchids (Paphiopedilum rothschildianum) using RAPD markers. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Random Amplified Polymorphic DNA CRAPO) markers were used to detect genetic variation in selected Taman Sabah's gennplasm collection of Paphiopedianum rothschildianum, an endangered slipper orchid in Sabah. Four populations of the species, which obtained from the locations including Penataran, Telupid, Kota Belud and Ampuan, were selected in the studied. A total of 46 amplified bands were scored from five RAPD markers and a mean of 9.2 per primer and the percentage of polymorphic bands was 93.48%. All populations of the species were found to share one or more RAPD markers with five primers. The cluster analysis of distance values was conducted to construct a dendrogram. The study revealed a moderate level of genetic polymorphism and variability among populations in P. rothschildianum and no direct relatonship between geographical distance and genetic variation between the four populations of P. rothschildianum.

Item Type: Academic Exercise
Uncontrolled Keywords: Slipper orchid, Paphiopedianum rothschildianum, Random Amplified Polymorphic DNA, Sabah Park, Penataran, Telupid, Kota Belud, Ampuan
Subjects: ?? QK495 ??
Divisions: SCHOOL > School of Science and Technology
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 17 Jan 2012 09:21
Last Modified: 23 Oct 2017 00:26
URI: http://eprints.ums.edu.my/id/eprint/3319

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