Extraction and screening of antioxidants in Metroxylon Sagu

Noor Idayu Mohd Tahir, (2004) Extraction and screening of antioxidants in Metroxylon Sagu. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Antioxidants were extracted from sago pith, sago processing effluent and waste sago pith obtained from a small-scale sago processing site in Beaufort with methanol to be further studied with phytochemical and biological activity tests while hexane was used to extract vitamin A and its precursors. The extracts were submitted to four phytochemical tests which detected flavonoids, tannin and phenolic compounds in the extracts. The concentration of flavonoids was contained highest in sago pith, followed by its waste water and the waste pith. Tannin presence was observed highest in the processing waste water. Biological activities of the extracts were assessed trough linoleic acid autoxidation test, peroxidation value determination test and brine shrimp lethality test. Sago pith showed the longest induction period of 5 days which indicates the highest antioxidant activity. The peroxidation value determination test showed that extract of waste water possess the highest capability of preserving the unsaturated fat from oxidizing, followed by sago pith extract and waste pith extract. Mortality percentage in brine shrimps incubated with different concentration of extracts was rather low, indicating that the extracts do not cause lethality in brine shrimps. Several phenolic compounds were observed after running silica gel 60 Fâ‚‚â‚…â‚„ column chromatography (acetone: water: acetic acid, 75:20:5, v/v) with subsequent thin layer chromatography (n-propanol:water, 64:36, v/v). Vitamin C was determined at 264 nm by using a C18 reversed-phase column HPLC with a mobile phase of acetonitrile: water (75:25, v/v) in which the highest concentration was observed in waste water, followed by sago pith and waste pith. Carotene was detected at 450 nm using UV/Vis spectrophotometer where sago pith contain the highest concentration, followed by waste pith and waste water extract. Sago pith and its byproducts (wastes) contain antioxidants and are non-toxic.

Item Type: Academic Exercise
Uncontrolled Keywords: Sago processing, Waste wago, Phytochemical, Biology activity, Metroxylon sagu, Beaufort, Sabah
Subjects: ?? QK710-899 ??
Divisions: SCHOOL > School of Science and Technology
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 26 Jan 2012 06:54
Last Modified: 23 Oct 2017 00:32
URI: http://eprints.ums.edu.my/id/eprint/3345

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