Srinivasa Jayachandran, (2007) Evaluation of the reproductive toxicity of diazinon in male and female rat offspring exposed to their mothers throughout pregnancy and lactation. PhD thesis, Universiti Malaysia Sabah.
Diazinon is an organophosphate insecticide widely used in agriculture. It is also known to have adverse health effects. Possible reproductive toxic effects are less studied. The aim was to study the possible reproductive adverse effects of the diazinon on rat offspring exposed in utero and during lactation. Dams were gavaged with diazinon at 0, 10, 15 and 30mg/kg/day prior to mating, during mating, pregnancy and lactation in separate groups. Maternal and reproductive outcome data and, male and female rat offspring reproductive parameter at puberty (PND70) and adulthood (PND 170) were examined. Male rat offspring were examined at puberty and adulthood for body weight, testis weight, epididymis weight, sperm count, motility and morphology, pituitary-gonadal hormone levels-FSH, LH, prolactin and testosterone, testicular marker enzymes activities-alkaline phosphatase, acid phosphatase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase and cholinesterase, qualitative and quantitative testicular and epididymal histology, total protein and Vitamin C levels and immunohistochemisty for 3 beta HSD. Similarly, female rat offspring were examined at puberty and adulthood for body weight, uterus weight, ovary weight, histological examination of ovary and uterus, pituitary-gonadal hormone levels, ovarian marker enzymes activities, ovarian total protein and Vitamin C levels and immunohistochemisty for 3 beta HSD. Diazinon caused a significant decrease in maternal body weight during gestation at 30mg/kg, but still there was an increase in body weight irrespective of the dose. The body weight, ovarian weight, uterus weight, plasma estradiol, prolactin and ovarian Vitamin C levels were decreased at postnatal day 22 in 30mg/kg dose dam-groups. 30 mg/kg dose induced significant adverse effects both at puberty and at adulthood in rat offspring. 30mg/kg diazinon dose, the male rat offspring at puberty showed a decrease in testicular weight, sperm count, motility, with an increase in percent abnormal sperm, degenerative changes with a decline in pituitary-gonadal hormones, 3 beta HSD and total protein level. Moreover, an increase in activity of alkaline and acid phosphatase was also observed. At adulthood, there was a decrease in testicular weight, sperm count, motility with an increase in percent abnormal sperm and a decrease in pituitary hormones, 3 beta HSD and total protein levels with an increase in testicular marker enzyme levels. Female rat offspring at puberty in 30 mg/kg dose showed a decrease in body weight, ovarian weight, pituitary-gonadal hormones levels, 3 beta HSD, total protein and Vitamin C concentration and an increase in ovarian marker enzymes levels. At adulthood, the female rat offspring exhibited a decrease in body weight, ovarian weight, pituitary-gonadal hormone levels, 3 beta HSD, G6PD activity, total protein and Vitamin C concentration with an increase in the activity of alkaline phosphatase, acid phosphatase and lactate dehydrogenase. There was evidence of some adverse reproductive effects at 15 mg/kg dose in both male and female rat offspring. The study showed that most of the adverse effects were irreversible and were evident at both puberty and adulthood in rat offspring, although a few parameters reverted back to the normal growth pattern. The degree of toxicity of diazinon on the male and female rat offspring was identical at puberty and adulthood. Overall, diazinon is a reproductive toxicant in both male and female offsprings when exposed during prenatal and postnatal life.
|Item Type:||Thesis (PhD)|
|Uncontrolled Keywords:||Reproductive toxicity, Diazinon, Rat offspring|
|Subjects:||R Medicine > RG Gynecology and obstetrics|
|Divisions:||SCHOOL > School of Medicine|
|Deposited By:||IR Admin|
|Deposited On:||02 Apr 2012 15:45|
|Last Modified:||02 Apr 2012 15:45|
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