Inhibiting two-component signal transduction system in mycobacterium tuberculosis

Jenifer Rolland L, (2005) Inhibiting two-component signal transduction system in mycobacterium tuberculosis. Universiti Malaysia Sabah. (Unpublished)

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Abstract

In this work, my aim is to identify potential antimicrobial compounds that inhibit the regulation of two-component system in Mycobacterium tuberculosis over osmotic stress. Few diversity of actinomycetes were isolated from a total of 16 soil samples that had been collected from the virgin tropical rainforest of Danum Valley in Sabah. The soil samples were mainly collected under selected trees in the Newbery Plot area. A total of 46 strains of actinomycetes had been isolated from these soil samples through isolation using HV media and purification using Oatmeal media. Through fermentation process, different secondary metabolites were extracted from all these actinomycetes strains. The resulting acetone extracts were screened for K� and Na� signals in Mycobacterium twocomponent signal transduction system, on media that had been developed to apply osmotic pressure. Mycobacterium smegmatis was grown using the modified M9 minimal media with low and high concentration of potassium (K�) and sodium (Na�)ions. Wildtype Mycobacterium smegmatis mc²155 strain H8000 was used as targeted microorganism in modified M9 minimal media. The pH of media was adjusted to 6.7. Glucose, thiamine hydrochloride (Vitamin B1) and the inoculum of M smegmatis were added into the media. The culture was incubated in 37°C for 3-4 days. Streptomycin was used as a negative control. A new simple but interesting discovery was found regarding streptomycin where its resulting inhibition zones were decreasing as the concentration of Na� signal in media arise. Besides reducing the growth of M smegmatis, higher concentration of signals in the media had increased the media density, which might explain why the fixed concentration of streptomycin used as control cannot widen its inhibition zone. Only Extract Hl1588 showed antimicrobacterial activity on the twocomponent signal transduction system in Mycobacterium. However, it was later defmed as toxic to M smegmatis because it showed inhibition zones on both low and high concentrations (Na� concentration: Low: 0.8% (w/v); High: 4.0% (w/v); K� concentration: Low 100 μM; High: 1 mM) of both studied signals.

Item Type: Academic Exercise
Uncontrolled Keywords: Soil, Antimicrobail, Oatmeal media, Mycobacterium tuberculosis, Lembah Danum, Sabah
Subjects: ?? S590-599.9 ??
Divisions: SCHOOL > School of Science and Technology
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 12 Jul 2012 04:29
Last Modified: 23 Oct 2017 06:53
URI: http://eprints.ums.edu.my/id/eprint/4504

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