Incidence of malaria in the interior division of Sabah, Malaysian Borneo, based on nested PCR

Vui, Clemente Michael Ling Wong and Ka , Lung Chong and Wan, Fen Joveen-Neoh and Lau, Tiek Ying (2011) Incidence of malaria in the interior division of Sabah, Malaysian Borneo, based on nested PCR. Journal of Parasitology Research, 2011 . ISSN 2090-0023

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Official URL: http://dx.doi.org/10.1155/2011/104284

Abstract

Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites. Methods. Nested PCR was used to detect the presence of human malaria parasites. A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study. Results. Nested PCR findings showed that there was no P. malariae infection while the highest prevalent malaria parasite was P. knowlesi, followed by P. vivax, P. falciparum, and mixed infection. Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results. Conclusion. The preliminary findings from molecular detection of malaria showed that P. knowlesi was the most prevalent Plasmodium species in the interior division of Sabah. The findings from this paper may provide a clearer picture on the actual transmission of different Plasmodium species in this region.

Item Type:Article
Uncontrolled Keywords:Small subunit ribosomal RNA, adolescent, Adult, Article, Blood analysis, Borneo, Child, Controlled study, Diagnostic accuracy, Female, Human, Incidence, Major clinical study, malaria, Malaria falciparum, Male, Mixed infection, parasite identification, parasite prevalence, Plasmodium falciparum, Plasmodium knowlesi, Plasmodium malariae, Plasmodium malariae infection, Plasmodium vivax, Plasmodium vivax malaria, polymerase chain reaction, priority journal, school child
Subjects:R Medicine > RA Public aspects of medicine
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:4613
Deposited By:IR Admin
Deposited On:24 Jul 2012 11:37
Last Modified:17 Feb 2015 15:04

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