Optimization of extraction time and temperature on antioxidant activity of Schizophyllum commune aqueous extract using response surface methodology

Yim, Hip Seng and Chye, Fook Yee and Rao, Vigneswara and Low, Jia Yin and Patricia @ Patricia Clementina Matanjun, and How, Siew Eng and Ho, Chun Wai (2013) Optimization of extraction time and temperature on antioxidant activity of Schizophyllum commune aqueous extract using response surface methodology. Journal of Food Science and Technology. pp. 275-283. ISSN 0022-1155

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Abstract

Central composite design of response surface methodology (RSM) was employed to optimize the extraction time (X1: 99.5-290.5 min) and temperature (X2: 30.1-54.9 °C) of Schizophyllum commune aqueous extract with high antioxidant activities and total phenolic content (TPC). Results indicated that the data were adequately fitted into four second-order polynomial models. The extraction time and temperature were found to have significant linear, quadratic and interaction effects on antioxidant activities and TPC. The optimal extraction time and temperature were: 290.5 min and 35.7 °C (DPPH• scavenging ability); 180.7 min and 41.7 °C (ABTS•+ inhibition ability); 185.2 min and 42.4 °C (ferric reducing antioxidant power, FRAP); 290.5 min and 40.3 °C (TPC). These optimum conditions yielded 85.10%; 94.31%; 0.74 mM Fe2+ equivalent/100 g; 635.76 mg gallic acid equivalent/100 g, respectively. The yields of antioxidant activities and TPC obtained experimentally were close to its predicted values. The establishment of such model provides a good experimental basis employing RSM for optimizing the extraction time and temperature on antioxidants from S. commune aqueous extract.

Item Type: Article
Uncontrolled Keywords: Antioxidant activity, Extraction time, Response surface methodology, Schizophyllum commune, Temperature, Total phenolic content
Subjects: ?? TP368-456 ??
Divisions: SCHOOL > School of Food Science and Nutrition
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 24 Oct 2012 00:09
Last Modified: 20 Oct 2017 06:06
URI: http://eprints.ums.edu.my/id/eprint/5139

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