Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats

Okazaki, Yasumasa and Kono, Isato and Kuriki, Takayoshi and Funahashi, Satomi and Funahashi, Soichiro and Iqbal, Mohammad and Okada, Shigeru and Toyokuni, Shinya (2012) Bovine lactoferrin ameliorates ferric nitrilotriacetate-induced renal oxidative damage in rats. Journal of Clinical Biochemistry and Nutrition, 51 (2). pp. 84-90. ISSN 0912-0009


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Milk provides a well-balanced source of amino acids and other ingredients. One of the functional ingredients in milk is lactoferrin (LF). LF presents a wide variety of bioactivities and functions as a radical scavenger in models using iron-ascorbate complexes and asbestos. Human clinical trials of oral LF administration for the prevention of colon polyps have been successful and demonstrated that dietary compounds exhibit direct interactions. However, antioxidative properties of LF in distant organs require further investigation. To study the antioxidant property of LF, we employed bovine lactoferrin (bLF) using the rat model of ferric nitrilotriacetate (Fe-NTA)-induced renal tubular oxidative injury. We fed rats with bLF (0.05%, w/w) in basal chow for 4 weeks and sacrificed them after Fe-NTA treatment. After intraperitoneal administration of 9.0 mg iron/kg Fe-NTA for 4 and 24 h, bLF pretreatment suppressed elevation of serum creatinine and blood urea nitrogen levels. In addition, we observed protective effects against renal oxidative tubular damage and maintenance of antioxidant enzyme activities in the bLF-pretreated group. We thus demonstrated the antioxidative effect of bLF against Fe-NTA-induced renal oxidative injury. These results suggest that LF intake is useful for the prevention of renal tubular oxidative damage mediated by iron.

Item Type: Article
Uncontrolled Keywords: Chemoprevention, Ferric nitrilotriacetate, Glutathione metabolism, Lactoferrin, Oxidative renal damage, Antioxidant, Creatinine, Lactoferrin, Nitrilotriacetate iron, Animal experiment, Animal model, Article, Colon polyp, Controlled study, Cow, Creatinine blood level, dietary intake, Enzyme activity, Kidney tubule damage, male, Nonhuman, Oxidative stress, Rat, Urea nitrogen blood level
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: ADMIN ADMIN
Date Deposited: 14 Nov 2012 09:03
Last Modified: 20 Oct 2017 07:00

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