Screen-printed histamine biosensors fabricated from the entrapment of diamine oxidase in a photocured poly(HEMA) film

Ching, Mai Keow and Fatimah Abu Bakar, and Abu Bakar Salleh, and Lee, Yook Heng and Rahman Wagiran, and Shafiquzzaman Siddiquee, (2012) Screen-printed histamine biosensors fabricated from the entrapment of diamine oxidase in a photocured poly(HEMA) film. International Journal of Electrochemical Science, 7 (5). pp. 4702-4715. ISSN 1452-3981


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A three-electrode system amperometric biosensor that consisted of screen-printed carbon paste working electrode (SPE), a commercial platinum rod counter electrode vs Ag/AgCl reference electrode was developed for rapid determination of histamine in prawns. The biosensor was designed from entrapping diamine oxidase (DAO) enzyme in a poly(2-hydroxyethyl methacrylate) (photoHEMA) film prepared via a simple and one-step direct photocuring process on a carbon paste screen-printed electrode (SPE). The photoHEMA film exhibited water absorption of 34.14% after four hours exposure to water and no leaching of DAO was observed from the hydrogel film. The histamine biosensor showed response time of < 50 s with a linear response range from 0 to 60 ppm histamine (R² of 0.9946). The sensitivity of the biosensor was of 5.56 nAppm ¯¹, with a limit detection of 0.65 ppm histamine. The performance of the fabricated biosensor in the analysis of histamine in tiger prawn (Penaeus monodon) samples was comparable to high performance liquid chromatography (HPLC). The three-electrode system was then converted to an all-screen-printed histamine biosensor by printing onto a polyester substrate with carbon paste to form the working and counter electrodes and Ag/AgCl paste as the reference electrode. The performance of all-screen-printed histamine biosensor was evaluated using potassium hexacyanoferrate (III) as a mediator deposited electrochemically on the carbon-paste SPE. The presence of this mediator demonstrated improvement to the response of the all-screen- printed histamine biosensor.

Item Type:Article
Uncontrolled Keywords:Diamine oxidase, Histamine, Photocurable methacrylate film, Screen-printed amperometric biosensor, Tiger prawn
Subjects:Q Science > QP Physiology
ID Code:5454
Deposited By:IR Admin
Deposited On:23 Nov 2012 11:09
Last Modified:23 Feb 2015 11:09

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