Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode

Shafiquzzaman Siddiquee, and Nor Azah Yusof, and Abu Bakar Salleh, and Soon, Guan Tan and Fatimah Abu Bakar, (2010) Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode. Journal of Solid State Electrochemistry, 16 . pp. 273-272.

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Official URL: http://dx.doi.org/10.1007/s10008-011-1322-y

Abstract

Electrochemical DNA biosensor was successfully developed by depositing the ionic liquid (e.g., 1-ethyl-3- methylimidazolium trifluoromethanesulfonate ([EMIM] [Otf])), ZnO nanoparticles, and chitosan (CHIT) nanocomposite membrane on a modified gold electrode (AuE). The electrochemical properties of the [EMIM][Otf]/ZnO/CHIT/ AuE for detection of DNA hybridization were studied. Under optimal conditions using cyclic voltammetry, the target DNA sequences could be detected in the concentration range of 1.0×10−⁻¹⁸ to 1.82×10¯⁴ mol L¯¹, and with the detection limit of 1.0×10−19 mol L−1. This DNA biosensor detection approaches provide a quick, sensitive, and convenient method to be used in the identification of Trichoderma harzianum.

Item Type:Article
Uncontrolled Keywords:DNA electrochemical biosensor, DNA hybridization, ZnO nanopaticles, Ionic liquid, Chitosan
Subjects:?? QD450-801 ??
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:5589
Deposited By:IR Admin
Deposited On:08 Jan 2013 08:36
Last Modified:17 Feb 2015 16:32

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