Separation and identi�cation of volatile compounds from liquid cultures of trichoderma harzianum by GC-MS using three different capillary colums

Shafiquzzaman Siddiquee, and Cheong, Bo Eng and Khanam, Taslima and Hossain Kausar, and Md. Mainul Hasan, (2012) Separation and identi�cation of volatile compounds from liquid cultures of trichoderma harzianum by GC-MS using three different capillary colums. Journal of Chromatographic Science, 50. pp. 358-367. ISSN 0021-9665

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Abstract

A simple, fast, repeatable and less laborious sample preparation protocol was developed and applied for the analysis of biocontrol fungus Trichoderma harzianum strain FA1132 by using gas chromatography-mass spectrometry. The match factors for sample spectra with respect to the mass spectra library of fungal volatile compounds were determined and used to study the complex hydrocarbons and other volatile compounds, which were separated by using different capillary columns with nonpolar, medium polar and high polar stationary phases. To date, more than 278 volatile compounds (with spectral match factor at least 90%) such as normal saturated hydrocarbons (C7-C30), cyclohexane, cyclopentane, fatty acids, alcohols, esters, sulfur-containing compounds, simple pyrane and benzene derivatives have been identified. Most of these compounds have not previously been reported. The method described in this paper is a more convenient research tool for the detection of volatile compounds from the cultures of T. harzianum.

Item Type: Article
Uncontrolled Keywords: Trichoderma harzianum strain FA1132, Gas chroma-tography–mass spectrometry, Hydro-carbons, Volatile compounds
Subjects: Q Science > QR Microbiology
Divisions: SCHOOL > Biotechnology Research Institute (BRI)
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 08 Jan 2013 00:31
Last Modified: 11 Oct 2017 01:39
URI: http://eprints.ums.edu.my/id/eprint/5590

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