Optimization of extraction time and temperature for antioxidant activity of edible wild mushroom, Pleurotus porrigens

Yim, Hip Seng and Chye, Fook Yee and Koo, Sze May and Patricia @ Patricia Clementina Matanjun, and How, Siew Eng and Ho, Chun Wai (2012) Optimization of extraction time and temperature for antioxidant activity of edible wild mushroom, Pleurotus porrigens. Food and Bioproducts Processing, 90 (2). pp. 235-242. ISSN 0960-3085


Download (50kB) | Preview


The extraction time and temperature of Pleurotus porrigens were optimized for the maximization of 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) radical cation inhibition activities, ferric reducing/antioxidant power (FRAP) and total phenolic content (TPC) using response surface methodology (RSM). A rotatable central composite design consisting of 14 experimental runs with three replicates at the central points was applied and second-order polynomial models were used to describe the experimental data regarding the responses. The experimental results adequately fitted into the second-order polynomial models with significant linear, quadratic and interaction effects of the independent variables. The optimized conditions were 372.8 min/32.0 °C (DPPH); 340.9 min/36.8 °C (ABTS); 240.0 min/38.1 °C (FRAP); and 310.1 min/43.6 °C (TPC) with corresponding yields of 32.66%; 91.21%; 7.91 mM Fe 2+ equivalent/100 g; and 494 mg gallic acid equivalent/100 g, respectively. The experimental values were close with those predicted values, indicating suitability of the model employing RSM for optimizing the extraction time and temperature on antioxidant activity from P. porrigens.

Item Type: Article
Uncontrolled Keywords: Antioxidant activity, Extraction time, Pleurotus porrigens, Response surface methodology, Temperature, Total phenolic content
Subjects: ?? QK474.8-495 ??
?? TP368-456 ??
Divisions: SCHOOL > School of Food Science and Nutrition
Depositing User: Unnamed user with email storage.bpmlib@ums.edu.my
Date Deposited: 11 Jan 2013 09:21
Last Modified: 11 Oct 2017 02:15
URI: http://eprints.ums.edu.my/id/eprint/5702

Actions (login required)

View Item View Item

Browse Repository
   UMS News
Quick Search

   Latest Repository

Link to other Malaysia University Institutional Repository

Malaysia University Institutional Repository