Polymorphisms of x-ray repair cross-complementing group 1 (xrcc1) in gastrointestinal cancer

Chiew, Jeniffer Lee Hwa (2008) Polymorphisms of x-ray repair cross-complementing group 1 (xrcc1) in gastrointestinal cancer. Universiti Malaysia Sabah. (Unpublished)

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Abstract

This research is done to identify the polymorphisms of x-ray repair crosscomplementing group I (XRCC 1) gene in gastrointestinal (GI) cancer. DNA was isolated from the blood samples, which were taken from Hospital Queen Elizabeth. The quality of DNA isolated was checked by spectrophotometer and were used for polymerase chain reaction. Polymerase chain reaction was carried out using a pair of primer specifically to amplify 517bp of the XRCC 1 gene that contained the Arg399G1n site. After the PCR, the amplified DNA was cut by using specific enzyme which will only cut at specific sequence of the DNA (Bcnl). After the restriction enzyme digestion, the result of restriction enzyme digestion was analyzed by using agarose gel electrophoresis in order to determine whether there is any polymorphism in the region of interest. From the result of the agarose gel electrophoresis, out of 10 samples, four samples with genotype frequency of 0.3481 were homozygote wild-type (Arg/Arg), three samples with genotype frequency of 0.17 were homozygote variant (Gln/Gln), and three samples with genotype frequency of 0.48 were heterozygote (Arg/Gln). However, this is just a preliminary result because we are not able to get large number of samples.

Item Type: Academic Exercise
Uncontrolled Keywords: polymorphisms of x-ray , gastrointestinal cancer
Subjects: Q Science > QM Human anatomy
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM FAUZIAH MATSIN
Date Deposited: 13 May 2013 04:04
Last Modified: 13 Oct 2017 09:03
URI: http://eprints.ums.edu.my/id/eprint/5909

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