Bacteria - sponge interaction: Existence of possible chemical correlation

Koh, Johleen Tse Boon (2010) Bacteria - sponge interaction: Existence of possible chemical correlation. Masters thesis, Universiti Malaysia Sabah.

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Abstract

The vast ocean has the reputation of having the greatest biodiversity. Therefore, represents an enormous resource for the discovery of chemotherapeutic agents. This particular investigation delves upon the study of bioactive compound from two marine sponges from coastal waters of Sabah, Amphimedon sp. and Xestospongia sp. and the most active sponge was then investigated for its relationship with its inherently available culturable bacteria in an effort to confirm the identity of the bioactive compound producer. All fractions except for fraction 1 and fraction 6 from the hexane crude extract from Amphimedon sp. showed antibacterial activity against 5 strains of marine environmental bacteria while all fractions except for fraction 1 from the 90% methanol crude extract showed antibacterial activity against 5 strains of marine environmental bacteria. Amphimedon sp. produced potent sterol as its bioactive compound, while Xestosponge sp produced aaptamine. Their identities were identified based on extensive Hl-NMR and MS data. Spongebacteria relationship was carried out by isolating culturable bacteria from sponge tissues, and a total of 40 strains of bacteria were isolated from Amphimedon sp. but only one strain (AA1) was found to have bioactive activity. 16S rRNA regions of AA1 were amplified using forward primer 27F (GAGTTTGATCCTGGCTCAG) while the reverse primer was JR1R (GACTACCAGGGTABCTAATC) and was compared with the top three matches provided by NCBI GenBank. Strain AA1 was found to be pro Bacillus sp. with 99% confidence and with e-value of zero. A mixture of fatty acids were isolated as active metabolite from AA1 and upon gas chromatography (GC) analysis and antibacterial assay with corresponding commercial fatty acids. It was confirmed that hexadecenoate was the active compound in AA1. In conclusion, culturable bacteria were not responsible for the production of active compounds in Amphimedon sp.

Item Type: Thesis (Masters)
Uncontrolled Keywords: bacteria sponge interaction, chemical correlation, bioactive compound, Amphimedon sp., culturable bacteria
Subjects: Q Science > QL Zoology
Divisions: SCHOOL > Borneo Marine Research Institute
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 09 Jul 2013 03:21
Last Modified: 11 Oct 2017 07:08
URI: http://eprints.ums.edu.my/id/eprint/6522

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