HPLC profiles comparison of leaf skin and leaf gel of aloe genus plant

Choo, Mei Siang (2005) HPLC profiles comparison of leaf skin and leaf gel of aloe genus plant. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Aloe vera is a popular traditional medicinal plant around the world. Extracts from both parts of the aloe leaf gel and leaf skin were obtained by using methanol as the extract solvent. These extracts were then partitioned by water and chloroform to separate polar and non-polar compounds. Four extracts were analysed under HPLC using Sulpercosil LC-I8 column (25cm x 4.6 mm I.D.). According to the analysis, the best elution programme needs to include both isocratic and linear gradient steps. Furthermore, while in the isocratic steps, lower flow rate, at O.5ml/minute, showed better resolution. Based on the parameters mobile phase solvent, acetonitrile showed better result than methanol. After the profiles optimizations, the best profiles for leaf gel and leaf skin were compared. The results showed most of the compounds in leaf gel and leaf skins were the same.

Item Type: Academic Exercise
Uncontrolled Keywords: aloe vera, medicinal plant, leaf gel, leaf skin, isocratic step, linear gradient step, acetonitrile
Subjects: T Technology > TD Environmental technology. Sanitary engineering
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 16 Jul 2013 05:34
Last Modified: 23 Oct 2017 07:09
URI: http://eprints.ums.edu.my/id/eprint/6565

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