Screening of microbial and plant extracts for new anti-Mycobacterium drugs

Ch'ng, Ai Ying (2007) Screening of microbial and plant extracts for new anti-Mycobacterium drugs. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Tuberculosis is one of the world's most pernicious disease that infected by Mycobacterium tuberculosis. Isocitrate lyase (ICL) is one of the enzymes in the glyoxylate metabolic pathway that is required in persistent infection and pathogenesis of M tuberculosis. In this study, a microbial acetone crude extract of actinomycete H7763 and 29 plant extracts of II plant species were screened using a cell-based screening system against the acetate and glucose utilization of Mycobacterium smegmatis mc²155, H8000. The H7763 acetone crude extract showed a wide partial inhibition zone (48 mm) on the acetate plate and no inhibition zone on the glucose plate. A partial purification of the H7763 crude extract was carried out by water-ethyl acetate extraction and the organic layer obtained was further purified using Reversed Phase High Performance Liquid Chromatography (RP-HPLC). Fractions collected from the RP-HPLC fractionation were screened in which the fractions of F7, F8, F9, Fl0, F11 and F12 showed positive results. For plant extracts, Alpinia galangal crude extract, Anacardium occidentale n-butanol extract and Chromolaena odorata n-butanol extract showed the most promising positive results. MIC of these plant extracts that prevented the growth of H8000 in the cell-based screening system was 1, 4 and 10 mgml�¹ respectively. However, these three plant extracts did not inhibit ICL activity at the low concentration of 1 and 2 mgml�¹. In conclusion, the ethyl acetate layer (F7, F8, F9, FlO, FIt and Ft2) of the H7763 acetone crude extract, Alpinia galangal crude extract, Anacardium occidentale n-butanol extract and Chromolaena odorata n-butanol extract had been shown to be potential inhibitors against the growth of Mycobacterium smegmatis mc²155, H8000.

Item Type: Academic Exercise
Uncontrolled Keywords: Mycobacterium tuberculosis, pernicious disease, enzyme, inhibitor, plant extract
Subjects: Q Science > QD Chemistry
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 29 Jul 2013 06:22
Last Modified: 23 Oct 2017 07:20
URI: http://eprints.ums.edu.my/id/eprint/6642

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