Expression of six chloroplast DNA genes in Jatropha Curcas callus under light and dark conditions

Chew, Cassandra Sze Yii (2011) Expression of six chloroplast DNA genes in Jatropha Curcas callus under light and dark conditions. Masters thesis, Universiti Malaysia Sabah.

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Abstract

The expression of genes encoded in the open reading frames of chloroplast genomes have been posited to be induced by light. The current study focused on the induction of ORFs encoded in the chloroplast genome of Jatropha curcas (Accession number FJ695500). J. curcas is an important non-edible oil seed crop, which produces oil with high calorific value and is regarded as a potential fuel substitute. By understanding the gene expression of chloroplast DNA under light stress, potential genes candidate can be used for callus transformation. Gene induction was characterized in leaves of the plant, green callus and white callus cultivated under condition of light and darkness. A total of six ORFs representing the genes YCF1, YCF2, psbD (photosystem II), rbcL (Rubisco), matK (Maturase K) and rpoC1(RNA polymerase) were targeted by designing speCific primers for their characterization. Specificity of primers was tested against the genomic DNA. Transcripts of six targeted genes were detected in all three replicates of the green and white callus under light and dark, except for ycf 2 gene in green callus under light. A part of gene ycf 2 was no transcribed using reverse transcription PCR, was then validated using real-time PCR assay. The reverse transcription PCR did not detect the gene amplified by YCFD primer in the green callus under photoperiod treatment. The ycf 2 gene located in the region of region 94050 to 95483 was posttranscriptional modified. In the real-time PCR assay, the amplification curves were produced from the amplification of all callus under different treatments, except for green callus under light.

Item Type:Thesis (Masters)
Uncontrolled Keywords:expression of gene, light, chloroplast, Jatropha curcas, fuel substitute
Subjects:Q Science > QK Botany
Divisions:SCHOOL > Biotechnology Research Institute (BRI)
ID Code:6669
Deposited By:IR Admin
Deposited On:31 Jul 2013 15:59
Last Modified:31 Jul 2013 15:59

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