Fermentation of groundnut shell enzymatic hydrolysate for fuel ethanol production by free and sorghum stalks immobilized cells of Pichia stipitis NCIM 3498

Ravindra Pogaku, and Ravinder, Rudravaram and Mangamoori, Lakshmi Narasu and Konakalla, Radhika and Chandel, Anuj Kumar and Gajula, Chandrasekhar (2011) Fermentation of groundnut shell enzymatic hydrolysate for fuel ethanol production by free and sorghum stalks immobilized cells of Pichia stipitis NCIM 3498. International Journal of Chemical Reactor Engineering, 9 (A61). ISSN 1542-6580

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Abstract

Groundnut shell (GS), after separation of pod, is readily available as a potential feedstock for production of fermentable sugars. The substrate was delignified with sodium sulfite. The delignified substrate released 670 mg/g of sugars after enzy- matic hydrolysis (50 C, 120 rpm, 50 hrs) using commercial cellulases (Dyadic Xylanase PLUS, Dyadic Inc. USA). The groundnut shell enzymatic hydrolysate (45.6 g/L reducing sugars) was fermented for ethanol production with free and sorghum stalks immobilized cells of Pichia stipitis NCIM 3498 under submerged cultivation conditions. Immobilization of yeast cells on sorghum stalks were con- firmed by scanning electron microscopy (SEM). A maximum of ethanol produc- tion (17.83 g/L, yield 0.44 g/g and 20.45 g/L, yield 0.47 g/g) was observed with free and immobilized cells of P. stipitis respectively in batch fermentation condi- tions. Recycling of immobilized cells showed a stable ethanol production (20.45 g/L, yield 0.47 g/g) up to 5 batches followed by a gradual downfall in subsequent cycles.

Item Type: Article
Uncontrolled Keywords: groundnut shell, enzymatic hydrolysis, sorghum stalks, immobilization, Pichia stipitis, ethano
Subjects: Q Science > Q Science (General)
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 19 Aug 2013 08:37
Last Modified: 17 Feb 2015 03:53
URI: http://eprints.ums.edu.my/id/eprint/6742

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