Polymerase Chain Reaction (PCR) analysis of mangrove soil microorganism from Kota Kinabalu City Bird Sanctuary

Clarice, Kian Kok (2005) Polymerase Chain Reaction (PCR) analysis of mangrove soil microorganism from Kota Kinabalu City Bird Sanctuary. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The objective of this study was to explore the diversity of the microbial community in soil sample from mangrove soils in Kota Kinabalu City Bird Sanctuary. Soil samples were collected from two different areas that were Avicenna (A) and landward (L) in Kota Kinabalu Bird Sanctuary. Two properties of the soil samples specifically pH and distributions of soil particulates were analyzed and this information was imperative in leading us to the type of microorganisms that might be discovered in the respective soil samples. Two direct DNA extraction techniques were employed in the research namely SDS and beat-beating method. From the comparison of two direct extraction methods, the SDS method was a better technique because of the higher quality and quantity of DNA that were obtained. The sample from the SDS based extraction was used in the PCR amplification. The PCR was performed using five set of primers which targeted at specific region of ribosomal DNA (rDNA). From the amplification, sample Avicenna contained bacteria and eukaryotes. In the sample of landward, the PCR product was obtained for sample bacteria, Pseudomonas and fungus.

Item Type: Academic Exercise
Uncontrolled Keywords: diversity, microbial community, mangrove soil, Kota Kinabalu City Bird Sanctuary, pH, DNA
Subjects: T Technology > TD Environmental technology. Sanitary engineering
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 26 Aug 2013 03:23
Last Modified: 23 Oct 2017 07:27
URI: http://eprints.ums.edu.my/id/eprint/6828

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