Antimicrobial activity of edible wild mushroom extracts

Lee, Slew Shia (2006) Antimicrobial activity of edible wild mushroom extracts. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The aim of this study was to examine the antimicrobial activity of SchizophyJlum sp. which is an edible wild mushrooms widely available and consumed by the local community in Sabah, Malaysia. In vitro studies using disk diffusion screening test and macrobroth dilution method have demonstrated antimicrobial activity of various extracts of SchizophyJlum sp. againts four foocIbome pathogenic bacteria such as Listeria monocytogenes L55, Salmonella lyphimurium 51000, Escherichia coli 0157: H7 imre 91/02c and Staphylococcus aureus 5277. The targeted extracts of Schizophyl/um sp. were methanolic extract, phenolic extract, flavonoid extract, terpenoid extract and\ essential oils. All the extracts were showing inhibitory effects againts different types of bacteris except for the terpenoid extract which showed no antimicrobial activity againts all the bacteria tested. The nmethanolic extract of Schizophy//um sp. inhibited the grownh of S. aureus, L. monocytoges and E. coli. The phenolic extract and flavonoid extracts were active against all the bacteria tested. Essential oils of Schizophyl/um sp. only showed antimicrobial properties against S. typhimurium and L. monocytogenes. Further analysis was carried out on the essential oils using GC-M5 has found out that l-octen-3-01 is the only compound present in the essential oils. The minimum inhibition (MIC) of various extract of Schizophyllum sp. were in the range of 90-264 mg/ml, where as the minimum bacteriocidal concentration (MBC) were in the range of 94- 268 mg/ml. The decimal reduction time for MBC ranged between 0.89-5.35 h, where as the decimal reduction time for MIC ranged between 1.71-29.58 h for the various extracts tested against selected bacteria.

Item Type: Academic Exercise
Uncontrolled Keywords: antimicrobial activity , SchizophyJlum sp., Salmonella lyphimurium , edible wild mushrooms
Subjects: T Technology > TX Home economics > Nutrition. Foods and food supply
Depositing User: MDM FAUZIAH MATSIN
Date Deposited: 30 Sep 2013 01:18
Last Modified: 17 Oct 2017 03:45
URI: http://eprints.ums.edu.my/id/eprint/7123

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