Anti-inflammatory activity of halogenated secondary metabolites of Laurencia snackeyi (Weber-van Bosse) Masuda in LPS-stimulated RAW 264.7 macrophages

Charles Santhanaraju Vairappan, and Kamada, Takashi and Won, Woo Lee and You, Jin Jeon (2013) Anti-inflammatory activity of halogenated secondary metabolites of Laurencia snackeyi (Weber-van Bosse) Masuda in LPS-stimulated RAW 264.7 macrophages. Journal of Applied Phycology, 25 (6). pp. 1805-1813. ISSN 0921-8971

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Abstract

Secondary metabolites of tropical seaweed are proven to exhibit variety of biological activities. Six species of seaweed (Caulerpa racemosa var. laete-virens, Caulerpa sertularioides f. longipes, Halymenia dilatata, Laurencia snackeyi, Padina boryana, and Sargassum swartzii) were tested for anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Crude L. snackeyi extract exhibit potent activity, and upon bioassay-guided isolation, it contained four halogenated compounds that exert profound inhibitory effects against nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. These compounds were subjected to spectroscopic measurements and were identified as palisadin A (1), aplysistatin (2), 5-acetoxypalisadin B (3), and palisol (4). Further experiments showed aplysistatin (2) to significantly inhibit NO production and prostaglandin-E2 (PGE2) production, and suppress inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression in LPS-stimulated RAW 264.7 cells. Therefore, aplysistatin (2) is suggested to inhibit NO and PGE2 production via the inhibition of iNOS and COX-2, indicating that its activity may be attributed to the modulation of anti-inflammatory agents.

Item Type: Article
Uncontrolled Keywords: Seaweed, Laurencia snackeyi, Halogenated metabolites, Anti-inflammatory
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > Institute for Tropical Biology and Conservation
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 15 Nov 2013 03:51
Last Modified: 11 Oct 2017 02:47
URI: http://eprints.ums.edu.my/id/eprint/7551

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