Determination of catechin, epica tecidn, theobromine, and caffiene compounds in various cocoa planting materials in Sabah

Mohana Kanapy Ganison, (2007) Determination of catechin, epica tecidn, theobromine, and caffiene compounds in various cocoa planting materials in Sabah. Universiti Malaysia Sabah. (Unpublished)

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Abstract

This study compares the quantity of the catechin, epicatechin, theobromine, and caffeine of cocoa beans from the various cocoa planting materials by using the reversed phase HPLC. Samples from different cocoa planting material were selected from the hybrid and clone varieties of cocoa pod. The cocoa fruits which contain the seeds were brought from the BAL cocoa plantation estate in Tawau. Only the ripe cocoa fruits were selected for the selected cocoa planting material. Cocoa beans were defatted before preparing the sample into two replicate. By using extraction method, catechin and epicatechin was extracted. Whereas theobromine and caffeine was extracted by using hot circulating bath. The result shows that theobromine compound for sample SCA9, BR25, PBC123, NA33, NA3 and mix hybrid are 2.43mg, 5.27mg, 2.90mg, 2.65rng, 5.34mg and 3.62mg, respectively. For caffeine, the amount of the compound in SCA9, BR25, PBC123, NA33, NA3 and mix hybrid are 0.520mg, 0.837mg, 0.385mg, 0.362mg, 0.610mg and 0.729mg respectively. Whereas for catechin, the compound in SCA9, BR25, PBC123, NA33, NA3 and mix hybrid are 133.26mg, 120.20mg, 13.41mg, 97.17mg, 7.302mg and 106.22mg, respectively. The result for the epicatechin cannot be identified because of systematic error occurred where there is no peak was identified in the graph obtained from the HPLC.

Item Type: Academic Exercise
Uncontrolled Keywords: cocoa bean, epicatechin, HPLC, extraction method, theobromine, quantity
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 01 Apr 2014 07:55
Last Modified: 10 Oct 2017 08:48
URI: http://eprints.ums.edu.my/id/eprint/8028

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