An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA

Clarence M. Ongkudon, and Sharadwata Pan, and Michael K. Danquah, (2013) An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA. Journal of Chromatography A, 1318. pp. 156-162. ISSN 0021-0673


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The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a chro- matographic technique that can handle larger DNAs. This paper report the development of a novel conical monolithic column format with pore and surface characteristics engineered for the isolation of 25 kbp DNA in a single step fashion. An effective method of eliminating wall channelling, a defect of most conventional monolithic chromatography systems which has caused significant loss of product, was applied to maximise DNA recovery. This method was based on a systematic reduction of wall channel size based on a predetermined correlation between column’s back pressure and wall channel size of a particular monolith pore size

Item Type: Article
Uncontrolled Keywords: Monolith , Chromatography , DNA isolation , Fosmid DNA
Subjects: Q Science > QH Natural history > QH301 Biology
Date Deposited: 01 Apr 2014 04:58
Last Modified: 11 Oct 2017 07:41

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