Isolation and characterization of bacteria from soils collected from the Schirmacher Oasis, Antarctica

Chee, Jo Anne (2007) Isolation and characterization of bacteria from soils collected from the Schirmacher Oasis, Antarctica. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Antarctica's drift towards South Pole 38 million years ago had allowed microorganisms to adapt to extreme conditions and thus, offer a unique diversity. The objective of this study is to isolate bacteria from soil samples obtained from Schirmacher Oasis, identify selected microorganisms by sequencing their 16S rDNA, and determine the relationship between these microbes by carrying out phylogenetic analysis. Bacteria were isolated by incubation of samples L43, L45, U5, West 2 and West 5 in MRS, Marine, Luria-Bertani, Streptomycetes and Antarctica Bacteria media. 77 six pure isolates were obtained and observed for their colony and cell characteristics. 45 of the isolates were Gram positive and 33 were Gram negative. Among 24 selected isolates, nine bacterial strains were psychrotolerant, 14 bacterial strains were mesophilic and one was undistinguished. RAPD analysis revealed that 23 out of the 24 strains were different. Ten isolates (C2, C4, H1, H3, H4, 11, 12, L8, L9 and N3) were identified based on their I6S rDNA sequences. The 16S rDNA genes were partially sequenced and the resulting sequence was analyzed using BLAST. Results revealed that C2 was from Micrococcus gen.; C4 from Arthrobacter gen.; H1 and 11 from Rhodococcu gen; H3 and H4 from Ralstonia gen.; J2 linked to Cohnella gen.; L8 from Streptomyces gen.;, L9 from Microbacterium gen;, and N3 from Bradyrhizobium gen. Phylogenetic analysis using ClustalW software indicated that these isolates can be classified into three groups: the first group consists of isolates C2, L8 and L9; the second group consists of isolates C4, 12, N3, H3 and H4; and the third group consists of isolates H1 and J1.

Item Type: Academic Exercise
Uncontrolled Keywords: isolate bacteria, Schirmacher Oasis, 16S rDNA, phylogenetic analysis, Antarctica Bacteria media
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 27 Jan 2014 05:16
Last Modified: 11 Oct 2017 04:38
URI: http://eprints.ums.edu.my/id/eprint/8055

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