Antioxidative and antimicrobial activities of M. denticula and M. tanarius

Bong, Wan Yuen (2007) Antioxidative and antimicrobial activities of M. denticula and M. tanarius. Universiti Malaysia Sabah. (Unpublished)


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The leaves of M denticula and M tanarius were extracted via reflux and the respective crude extracts were then fractionated using solvent-solvent extraction with 4 solvent with different polarity which yielded hexane (HEX), dichloromethane (DCM), n-butanol (BUT), and methanol aqueous (AQ) extracts. Total phenolic content, antioxidative and antimicrobial activities of the extracts were evaluated. The total phenolic content, determined according to the Folin-Ciocalteu method was varied between 115.58 mg GAE/g and 204.94 mg GAE/g. Antioxidative activities of the extracts were measured using the DPPH method. The antioxidative activities were then compared with that of butylated hydroxytouJene (BHT). The results showed that BUT extract of M tanarius had the highest antioxidative activities among the fractions tested with the 64.23 % inhibition at the highest concentration (0.04 mg ml�¹) tested. These results showed strong association with the total phenolic content (r² = 0.9684), suggested that phenolic compounds are probably responsible for the antioxidative activity of the Macaranga sp. The antimicrobial activity of the extracts, which evaluated by the disc diffusion assays showed weak inhibition against the bacteria (8 aureus, B. cereus, P. aeruginosa, S. typhimurium and E. coli). Conclusively, M denticula and M tanarius extracts tested possesses antioxidative activities which rendered them suitable as potential therapeutics, thus made them excellent candidates for more detailed investigation.

Item Type: Academic Exercise
Uncontrolled Keywords: M. denticula, M. tanarius, extract, phenolic, reflux, solvent-solvent extraction, antioxidative, antimicrobial activity
Subjects: Q Science > QD Chemistry
Divisions: SCHOOL > School of Science and Technology
Date Deposited: 29 Jan 2014 03:25
Last Modified: 11 Oct 2017 04:46

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