Biological activities of inhibitor of malate synthase in the glyoxylate pathway of mycobacterium

Khoo, Yau Liang (2007) Biological activities of inhibitor of malate synthase in the glyoxylate pathway of mycobacterium. Universiti Malaysia Sabah. (Unpublished)


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Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. Anti-tuberculosis drugs that are currently available preferentially targeting bacteria during active growth and replication, and requiring long treatment times to successfully clear a TB infection. Glyoxylate shunt or cycle is one of the targets resulting in either growth inhibition or death of the bacteria. Therefore, malate synthase (MLS) in glyoxylate cycle has become a new drug target, particularly for the latent infection. The focus of this study was to isolate new malate synthase inhibitors from a strain of actinomycetes, H7763. The fractions were obtained from the bioactive crude acetone extract of H7763 using solvent-solvent extraction and RP-HPLC techniques and tested against malate synthase using (MLS) enzymatic inhibitory assay. The aqueous layer of the extraction of H7763 was found to produce an active fraction (Fraction 7) based on a fractionation condition. The fraction 7 of H7763 was water soluble and gave a partial inhibition zone only in the acetate plate against M.smegmatis mc²155, H8000. The fraction 7 was partially pure as analyzed using RP-HPLC which was assayed for its inhibitory activity using a malate synthase (MLS) enzymatic assay. The results showed a decreased in enzyme and specific activities of H8000 when double volume of the initial concentration of the fraction 7 was added against malate synthase (MLS) of H8000. Therefore, fraction 7 of H7763 can be considered as an enzyme inhibitor of glyoxylate cycle.

Item Type: Academic Exercise
Uncontrolled Keywords: Mycobacterium tuberculosis, infection, Glyoxylate shunt, malate synthase, enzyme inhibitor
Subjects: Q Science > QR Microbiology
Divisions: SCHOOL > School of Science and Technology
Date Deposited: 06 Mar 2014 08:32
Last Modified: 11 Oct 2017 04:55

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