Proliferation of Phaleonopsis g;gantea protocorm segments on NDM media supplemented with 6-benzylaminopurine (BA)

Lai, Hui Ting (2007) Proliferation of Phaleonopsis g;gantea protocorm segments on NDM media supplemented with 6-benzylaminopurine (BA). Universiti Malaysia Sabah. (Unpublished)

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Abstract

This research was conducted to determine the optimum level of the hormone cytokinin 6- benzylaminopurine (BA) for the maximum proliferation of P. gigantea protoconn segments. NDM as the basal medium was supplemented with 11 different concentrations of hormone BA (0, O.S, 1.0, 1.5, 2.0, 2.S, 3.0, 3.S, 4.0, 4.S and 5.0mg/I BA). Each medium was replicated 4 times with 10 protocorm segments per replicate. The experiment was carried out for 84 days in the Tissue Culture Laboratory of VMS by using complete random experimental design (CRD). From the result obtained, the average percentage protocorm segments proliferated were approach to maximum after 70 days of culture initiated. However, the statistical analysis did not show significant different between the treatments. After 70 days of culture, high percentage of proliferation (40%) occurred in TS (NDM+2.0mg/I BA) while only (10%) proliferated in T9 (NDM+4.0mg/I BA). PLBs formed per segment in TS (NDM+2.0mg/I BA) ranged 17-36 after 70 days of culture. In the experiment, T2 (NDM+0.5mg/I BA) also showed high percentage of protocorm segments proliferated (37.50%) after 70 days of culture, however high percentage of dead segments (20.00%) also occurred in this medium.

Item Type: Academic Exercise
Uncontrolled Keywords: cytokinin 6-benzylaminopurine, complete random experimental design, P. gigantea, Tissue Culture Laboratory, protocorm segment
Subjects: Q Science > Q Science (General)
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 20 Feb 2014 05:23
Last Modified: 12 Oct 2017 00:08
URI: http://eprints.ums.edu.my/id/eprint/8231

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