Development of a protocol for the immobilization of pyrodinium bahamense var. compressum for use as a biosensor for harmful algal blooms

Yeoh, Jason (2006) Development of a protocol for the immobilization of pyrodinium bahamense var. compressum for use as a biosensor for harmful algal blooms. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Harmful Algal Bloom (HAB) algae species that is found in Sabah, Pyrodinium bahamense var. compressum was immobilized and cultured in 4 types of different culture media in order to determine the growth rate after it had been immobilized. Pyrodinium bahamense var. compressum cells were immobilized through gel-entrapment technique by using sodium alginate (3% w/v). Beads with the diameter from 4 ± 0.33 rom until 5 ± 0.33 nun is formed with 10 ml of algae-alginate mixture were able to produce 100 beads. These beads were cultured in 4 types of media that were f/2, f/50, Ll 10 dan Ll 50 at the temperature of 25 ± 3°C and 14:10 light:dark cycle with light intensity of 3000 lux for 21 days. Difference between growth rate of free algal cells culture and immobilized algal cells culture was observed through cell density per day value and OD reading at 680 run wavelenght per day value with the growth rate of immobilized algae cells was slower compared to the growth rate of free algal cells. Overall, cell density value is proportional to OD reading at 680 nm wavelength value for all culture media for immobilized algal cells culture with the growth rate of immobilize algal cells were optimum when cultured in f/2 media.

Item Type: Academic Exercise
Uncontrolled Keywords: Harmful Algal Bloom, growth rate, algal cells culture, cell density, immobilized algal cell
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 28 Feb 2014 02:54
Last Modified: 12 Oct 2017 03:59
URI: http://eprints.ums.edu.my/id/eprint/8313

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