Study of polvphenols in brown algae

Tan, Kai Yee (2008) Study of polvphenols in brown algae. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Marine brown algae are known to produce various secondary metabolites such as terpenes, steroids, and polyphenols. It is also lmown that phloroglucinol-based polyphenols (phlorotannins) are found only in brown algae. Present investigation delves upon the chemical profiling, phenolic quantification and bioactive potential assessment (antibacterial and antioxidant activity) in four species of brown algae (Surgussum sp., Pudina sp., Turbinaria sp., ami Dictyota sp.). Results from chemical profiling revealed all brown algae contain phlorotannins and various non-polar metabolites. Dictyota sp. had more compounds compared to the other three species of brown algae. Phenolic content for CHCI₃ extracts of four species of brown algae were recorded at the range of 19-37 μg P per gram of partially dried seaweed; EtOAc extracts was recorded at the range of 27-40 μg P per gram of partially dried seaweed. Phenolic content was highest in Dictyota sp. < Padina sp. < Turbinaria sp. < Sargassum sp. Results obtained by DPPH assay was comparable with FRAP assay. Percentage of scavenging from DPPH assay indicated highest antioxidant potential in Sargassum sp. < Padina sp. < Turbinaria sp. < Dictyota sp. while FRAP assay indicated Dictyota sp. < Sargassum sp. < Turbinaria sp. < Padina sp. Among the four species of brown algae, the range of percentage radical scavenging from DPPH assay was 12-35% and the range of FRAP was 4.8- 9.5 mg AA/g. All four species possessed antibacterial properties when tested with environmental and pathogenic microbes most notably Sargassum sp. and Dictyota sp.

Item Type: Academic Exercise
Uncontrolled Keywords: Marine brown algae, phloroglucinol-based polyphenol, phlorotannin, phenolic, pathogenic microbes
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 27 Feb 2014 01:35
Last Modified: 12 Oct 2017 04:15
URI: http://eprints.ums.edu.my/id/eprint/8347

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