Study on antibacterial effect of fungus, termitomyces on different types of bacteria

Jagedeswari Marriappan, (2008) Study on antibacterial effect of fungus, termitomyces on different types of bacteria. Universiti Malaysia Sabah. (Unpublished)

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Abstract

This study was carried out to identify antibacterial properties of fungus Termitomyces in form of mycelia culture and conidial extraction. The objectives of this study were to evaluate the antibacterial potential of fungus, to compare the antibacterial effect of fungus Termitomyces, in the form of mycelia culture and conidia extraction and also to find out the lowest concentration of the extraction of conidia and mycelia culture that will inhibit the growth of the tested bacteria. A total of 10 sample each from mycelia and conidial extraction was tested against Bacillus cereus (gram +ve), Escherichia coli (gram -ve) and Staphylococcus aureus (gram +ve). Anti-bacterial activity of the extract was determined using disk-diffusion method (Kirby-Bauer) and minimum inhibitory concentration (MlC) was carried out to identify the lowest concentration needed to inhibit the growth of selected bacteria. Serial dilution method was used with dilution of 101 till 105 for the MIC. Mycelia and conidial extraction of the tested Termitomyces showed almost same spectrum of antibacterial activity. The highest antibacterial inhibitory activity (±5.3mm) was recorded for the conidial extraction of Termitomyces against Escherichia coli. The second widest zone of inhibition (±4.3mm) was recorded for the conidial extraction tested against Bacillus cereus. All the extraction of mycelia and conidial have an inhibition zone against tested bacteria. The inhibition zone of the conidial extraction was higher compared to the mycelia extractions expect for Staphylococcus aureus (±1.5mm). The minimum inhibitory concentration (MIC) of mycelia extraction tested against bacteria showed higher antibacterial inhibitory activity compared to the conidial extraction. For the MIC of the conidial extraction, the inhibition properties stopped at dilution of 10³. This indicates that increase in dilution of the concentration of the extraction decreases the capability to inhibit the growth of selected bacteria decreases. In conclusion, Termitomyces is believed to have antibacterial properties against tested bacteria.

Item Type: Academic Exercise
Uncontrolled Keywords: conidial extraction, mycelia culture, Termitomyces, fungus, Serial dilution method, minimum inhibitory concentration
Subjects: Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 26 Feb 2014 08:17
Last Modified: 12 Oct 2017 05:24
URI: http://eprints.ums.edu.my/id/eprint/8361

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