Determination of aspartame, benzoic acid and caffeine in soft drinks using high performance liquid chromatography

Wong, Tsyr Min (2008) Determination of aspartame, benzoic acid and caffeine in soft drinks using high performance liquid chromatography. Universiti Malaysia Sabah. (Unpublished)

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Abstract

A simple and rapid analysis of soft drinks by high performance liquid chromatography with diode array detector allows the simultaneous determination of aspartame. benzoic acid and caffeine. The separation was performed on an Agilent Zorbax Eclipse XDBC 18 (5 μm, 4.6 x 150 mm) column at 50°C within 5 min at 1.0 mL/min flow rate by an isocratic elution with acetonitrile (0.05 % TF A) and ionized water (0.1 % TFA) (20:80, v/v). The determination was set at 210 nm, 230 nm and 280 nm for aspartame. benzoic acid and caffeine respectively. The optimization on mobile phase polarity and flow rate show a significant effect on retention time and peak height signal, while temperature shows significantly small changes and can be ignored. The retention time for caffeine, aspartame, and benzoic acid were at 2.155 min. 2.810 min and 4.139 min respectively. Good linearities (r²=.0999) between concentration of all analytes and relevant peak area and peak height responses were achieved over the range 100 - 500 ppm. All samples used for analysis requires minimal sample treatment. Aspartame only found in Pepsi Original at 88.11 mg in each bottle of 355 mL. Amount of benzoic acid in Pepsi Twist and Pepsi Max were 8.18 mg and 23 .754 mg respectively in each bottle of 355 mL not found in Pepsi Original. Amount of caffeine contain in each bottle of 355 mL soft drink were 31.19 mg in Pepsi Original, 33.41 in Pepsi Max and 38.03 mg in Pepsi Twist. The observed level of additives in soft drink samples are significantly lower than the permitted level stated in Malaysia Food Regulation

Item Type: Academic Exercise
Uncontrolled Keywords: soft drink, flow rate, mobile phase polarity, Aspartame, Agilent Zorbax Eclipse, benzoic, acid, caffeine, liquid chromatography
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 27 Feb 2014 03:05
Last Modified: 12 Oct 2017 05:26
URI: http://eprints.ums.edu.my/id/eprint/8365

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