Screening for mitogen-activated protein kinase kinase 1 (MKKI) inhibitor in fungi

Jessica Peter, (2005) Screening for mitogen-activated protein kinase kinase 1 (MKKI) inhibitor in fungi. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Microfungi of varied species were isolated from soil samples collected under leaf litters of trees in the Newberry research plot situated in Danum Valley Conservation Area. These fungi were isolated using potato-dextrose agar (PDA) with the addition of 0.75% chloramphenicol and sodium chloride, NaCI, (0.005%) which will selectively inhibit the growth of other microbes other than fungus. The fungi were then purified on the same media, but without the 0.75% chloramphenicol and 0.005% NaCI, to obtain pure single colony of the fungi. Morphological observations, fermentation for secondary metabolites and stocking in silica gel for long term storage of the fungi were made during this time. Acetone extracts of the fungi were then tested in the MKK 1 p³�� inhibitor screening system. The screening system utilizes mutant yeast MKKl p³�� which is under the control of the strong GAL 1 p. Overexpression of the mutant gene will cause cell growth arrest with the addition of galactose. So far, 119 fungal extracts (26 extracts were provided by Mr. Ong Si Mon, 36 extracts provided by Mr. Vun Su Chiun and 57 extracts isolated from my soil samples) have been tested and none have showed any activity on the system. There was no growth on the galactose plate around the disc extracts and no inhibition zone in the glucose plate discs extracts. All the 57 fungal isolates present various diversity. Sectoring has been observed in some of the fungal strain, H9434 (DV234-1) and H9473 (DV283-2), isolated from Danum Valley, suggesting genetic instability of the fungi

Item Type: Academic Exercise
Uncontrolled Keywords: Danum Valley Conservation Area, microfungi, Newberry research plot, Acetone extract, mutant gene, overexpression
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 13 Mar 2014 07:01
Last Modified: 12 Oct 2017 06:17
URI: http://eprints.ums.edu.my/id/eprint/8475

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