Quantification of sakuranetin in paddy leaves and stems after elicitation with abiotic elicitors (UV, AgNO₃, CuSO₄).

Ratnavell Muniandy, (2007) Quantification of sakuranetin in paddy leaves and stems after elicitation with abiotic elicitors (UV, AgNO₃, CuSO₄). Universiti Malaysia Sabah. (Unpublished)

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Abstract

Phytoalexins are substances produced in appreciable amounts in plants only after stimulation of various biotic or abiotic agents. Sakuranetin is one type of phytoalexin that yielded by paddy. Sakuranetin was extracted from paddy leaves and stems after elicitaled by silver nitrate. cuprum (II) sulfate and UV irradiation. The extraction was done by using methanol 70% twice and concentrated in vacuo, Then, the concentrated samples were re-extracted three times using diethyl ether and dried using evaporator. TLC was conducted and sakuranetin detected at Rf 0.09 under 365 nm UV light. Sakuranetin scraped and eluted using methanol. Extracted compound subjected to spectrophotometry at 337 nm. Concentration of sakuranetin present in sample was vcalculated. Elicitation by silver nitrate signifi cantly accumulated the highest amount of sakuranetin in leaves of paddy followed by elicitation by UV radiation and cuprum sulfate. However, elicitation by UV radiation and silver nitrate in paddy stems produced significantly highest amount of sakuranetin. Comparing sakuranetin amount recovered from both leaves and stems, leaves appeared to be significantly higher than that recovered from stems. There were fewer studies about sakuranetin as alleopathic chemical in paddy plants. Further researches should be conducted on effectiveness of sakuranetin as alleopathic chemical, natural herbicides and its antimicrobial activity.

Item Type: Academic Exercise
Uncontrolled Keywords: Phytoalexins , Sakuranetin , UV irradiation , paddy leaves and stems
Subjects: S Agriculture > SB Plant culture
Depositing User: MDM FAUZIAH MATSIN
Date Deposited: 01 Apr 2014 04:56
Last Modified: 13 Oct 2017 01:48
URI: http://eprints.ums.edu.my/id/eprint/8640

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