Screening selected flowers for potential antibacterial activity

Ling, Jing Jing (2007) Screening selected flowers for potential antibacterial activity. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The objectives of this study were to extract the selected flowers with methanol, evaluate their antibacterial activity against four standard bacteria by using disc diffusion method and compare the antibacterial activity of the flowers, The ten species of flowers used in this study were Yellow allamanda (Allamanda cathartica). Madagascar periwinkle (Catharanthus roseus). Crown-of-Thoms (Euphorbia milli), Bachelor's button (Gomphrena globosa), Hibiscus (Hibiscus rosa sinensis), Spider lily (Hymenocallis littoratis), Mile-A-Minute Vine (Ipomoea eairica), Lantana (Lantana camara), Toothache plant (Spilanthes acmella) and Crepe jasmine (Tabernaemonrana divaricata). The methanolic extracts of the flowers were screened for antibacteriaJ activity against Escherichia coli, Salmonella thyphimurium, Staphylococcus aureus and Pseudomonas aeruginosa. The screening on the flowers revealed. varying degree of antibacterial activity against various pathogenic bacteria. All the flower samples except Bachelor's button showed clear inhibition zone as a result of antibacterial activity towards S. aureus. The flower sample that was identified showing the significant antibacterial activity against S. allreus was Crown-of-Thorns, with the inhibition diameter of 4.33 mm. Yellow allwnanda, Madagascar periwinkle, Mile-A-Minute Vine, Toothache plant and Crepe jasmine showed the same diameter of 2.00 mm. Spider lily showed inhibition of 1.80 mm. Hibiscus exerted 1.00 mm diameter of inhibition while Lantana showed the smallest inhibition zone of 0.50 mm. However, the entire 10 sample flowers did not show any inhibition zone as a result of antibacterial activity towards Escherichia coli. Six flower samples exhibited inhibition zones as a result of antibacterial activity towards Salmonella thyphimurium. From the result, Spider lily, Toothache plant and Crepe jasmine showed the same inhibition zone with diameter of 1.50 mm. Madagascar periwinkle, Hibiscus and Toothache plant were less resistant to S. lhyphimllrium as it exened 1.33 mrn, 0.67 mm and 1.00 mm diameter of inhibition zone respectively. The remaining flower samples did not exert any clear zone. There were 5 out of 10 flower samples that inhibited the positive results with the Pseudomonas aeruginosa tested for the antibacterial activity. There were no obvious differences between the tested plant samples of Hibiscus and Toothache plan~ showing the same inhibition diameter of 1.00 mm. Madagascar periwinkle and Spider lily both showed 1.17 rom of inhibition diameter. Crown-of-Thoms showed the largest inhibition zone of 1.50 mm. The remaining flower sample revealed negative results. In conclusion, the antibacterial test showed the most significant result in S. aureus with the flower named Crown-of-Thorns, with the mean diameter of 4.33 mm.

Item Type: Academic Exercise
Uncontrolled Keywords: flowers with methanol, standard bacteria , disc diffusion method , antibacterial activity , Pseudomonas aeruginosa
Subjects: Q Science > QK Botany
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM FAUZIAH MATSIN
Date Deposited: 02 Apr 2014 02:17
Last Modified: 13 Oct 2017 02:00
URI: http://eprints.ums.edu.my/id/eprint/8647

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