Quantification of rishitin in tomato leaves and stems after elicited by biotic and abiotic elicitors

Jaya Suria Arul Sebastian Micheal, (2007) Quantification of rishitin in tomato leaves and stems after elicited by biotic and abiotic elicitors. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Levels of rishitin, a stress metabolite of tomato plant (Lycopersicon esculentum Mill.), were monitored in the leaves and stems of nine weeks old plant which were elicited with 1% silver nitrate and Xanthomonas campestris pv. oryzae. Extraction using 95% ethanol and 100% chloroform were followed by isolation via thin layer chromatography method. Quantification of rishitin in each plant part after each treatment was carried out using spectrophotometer. The experiment on leaves and stems of tomato plants treated with silver nitrate and Xanthomonas campestris pv. oryzae. produced an insignificant result for all possible combinations of elicitors and plant parts elicited except for the elicitation on tomato leaves. Two independent samples Mann-Whitney test illustrated that both plant parts and both elicitors are independent and not influenced by each other. Thus, study regarding the relationship between different plant parts and their impact on rishitin production under different treatments is crucial in order to improve plant defense system in the near future.

Item Type: Academic Exercise
Uncontrolled Keywords: rishitin, tomato plant, isolation, chromatography method, defense system
Subjects: S Agriculture > SB Plant culture
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 19 May 2014 02:21
Last Modified: 13 Oct 2017 02:33
URI: http://eprints.ums.edu.my/id/eprint/8736

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