Isolation of bioactive compounds extracted from Eurycoma longifolia Jack old leaves, screening for anti-bacterial, anti-fungal and GSK-3beta inhibition

Ho, Chun Loong (2006) Isolation of bioactive compounds extracted from Eurycoma longifolia Jack old leaves, screening for anti-bacterial, anti-fungal and GSK-3beta inhibition. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The plant Eurycoma longifolia Jack or known more commonly as Tongkat Ali has long been a source of traditional remedy curing infertility and also to treat mild illnesses like dysentery and rashes. In this study, the screening and isolation of compounds from Eurycoma longifolia Jack old leaves, by extraction using solvent-solvent method and chromatography methods, have been tested for anti-bacterial, antifungal and Glycogen Synthase Kinase-3beta inhibition activity. The separations of the phytochemicals were performed by liquid-liquid extraction to allow separation according to its classes and specific characteristics. It was determined from the test that the leaf extracts showed activity against Escherichia coli, Staphylococcus aureus and Candida krusei, but had no activity against Bacillus subtilis, Candida albicans or GSK-3beta The study includes isolation via Column Chromatography and verification by Thin Layer Chromatography. It was shown that the leaf extract possesses activity at possibly a single compound against E. coli, two possible compounds showing activity against C. krusei and 7 possible compounds against s.aureus. It was assumed that during isolation a single compound was collected at a specific time in these compound and further study in the future. The comparison of the leaf activity to the test performed using the root and the bark of the plant had also been performed and it was proved that the plant has no activity against GSK-3beta, but the leaf may have precursors in producing even more effective anti-microbial drugs. The experiment had proven that chemicals exhibiting anti-bacterial and antifungal exist in the plants and had been purified to a certain extent.

Item Type: Academic Exercise
Uncontrolled Keywords: Tongkat Ali, screening, isolation, extraction, solvent-solvent method, chromatography method, GSK-3beta
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 19 May 2014 02:23
Last Modified: 13 Oct 2017 02:36
URI: http://eprints.ums.edu.my/id/eprint/8738

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