Screening for mannanase activities of bacteria from soil and water samples obtained from maitri, antarctica and around Sabah

Tam, Heng Keat (2006) Screening for mannanase activities of bacteria from soil and water samples obtained from maitri, antarctica and around Sabah. Universiti Malaysia Sabah. (Unpublished)

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Abstract

Palm kernel cake is an important ingredient for the formulation of animal feed but it has a high percentage of hemicellulose (mannan) contents that hardly consumed by nonruminant animals. In addition, mannan is also an anti-nutritive agent that prevents the nutrients in PKC to be fully absorbed by poultry when used as feed. Mannan can be removed by mannanases to improve nutrition. Mannanases are produced by plants, fungi and bacteria. The objectives of this project are to screen and identify mannanaseproducing bacteria obtained from Maitri, Antarctica and around 8abah. Bacteria were isolated and grew on various agars. Isolated bacteria were subjected screening for mannanases using 0.15% guar gum labeled Remazol Brilliant Blue (RBB) or 0.15% locust bean gum labeled RBB with yeast extract, polypeptone, ammonium nitrate, potassium phosphate monobasic and magnesium chloride. Haloes formation is visible if bacteria have a mannanase activity. Mannanase positive bacteria were differentiated using RAPD-PCR with 9 pirmers (A10, All, A13, A17, El, E3, E4, E5, and E6). Only primers E3 and E5 produced sufficient data to differentiate these bacteria. From the RAPD data, 12 different bacteria strains from tropics and four different strains from Antarctica were obtained. Five bacteria strains from tropics and five strains from Antarctica bacteria including one non-mannanase producing bacterium with fungi-liked characteristic were later identified based on their 168 rDNA gene sequences. Tropical bacteria were amplified using primers B8F 8/20 and B8R 1541120 while Antarctic bacteria with primers FrvF and FrvR. 168 rDNA of two of terrestrial bacteria and three of the Antarctica bacteria were cloned onto pCR 2.1-TOPO vector and sequenced. Tropical bacteria that produce mannanase were identified as Streptomyces sp. and Paenibacillus amylolyticus. Mannanase producing bacteria that isolated from Antarctic bacteria were identified as Arthrobacter sp. and Arthrobacter oxydans. Additionally, a non-mannanase producing bacterium was identified as Bacillus cereus.

Item Type: Academic Exercise
Uncontrolled Keywords: palm kernel cake, mannan, anti-nutritive agent, Remazol Brilliant Blue, nutrition
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 21 Apr 2014 03:03
Last Modified: 13 Oct 2017 03:06
URI: http://eprints.ums.edu.my/id/eprint/8792

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