Analysis of proteins in rice using capillary electrophoresis

Linnga Rajan Peranjisi, (2008) Analysis of proteins in rice using capillary electrophoresis. Universiti Malaysia Sabah. (Unpublished)

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Abstract

The analysis of protein in Sabah local rice varieties was a pioneer research in order to study the protein fractions or composition in it. The local rice varieties that had been used in this study were Huma rice, Bario rice, White Glutinous rice and Black Glutinous rice. Each of the rice samples was extracted into its protein fractions: albumin, globulin, glutelin and prolamin according to its solubility by using Osborne Fractionation method. The protein fractions were analyzed using Capillary Electrophoresis (CE). Each fraction was analyzed in a 30 cm uncoated fused silica capillary at 25°C with zero pressure, voltage of 5kV and detected by UV detector at wavelength 214nm. Capillary cleaning protocols were carried out before and after analysis. Each fraction was analyzed using two different buffers at two different concentrations and pH. 100mM Tris-HCI buffer at pH 7.00 gave the sharper peak and less stacking compared to 50mM phosphate buffer. Among the four types of local rice, Huma rice and Bario exhibited a high proportion of glutelin followed by White Glutinous rice and Black Glutinous rice whereas globulin can be said as second major protein component in the local rice varieties. The abundance of albumin and prolamine in the local rice varieties are relatively low. Huma rice exhibited most of the peaks for its protein fraction whereas White Glutinous rice exhibited two peaks for its protein fraction, globulin and glutelin. The most hydrophobic protein, prolamin, in which theoretically should be less in rice, exhibited a peak as one of the protein components in Black Glutinous rice despite albumin, globulin and glutelin.

Item Type: Academic Exercise
Uncontrolled Keywords: protein fraction, local rice, Capillary Electrophoresis, UV detector, pH
Subjects: T Technology > TP Chemical technology
Divisions: SCHOOL > School of Science and Technology
Depositing User: MDM SITI AZIZAH IDRIS
Date Deposited: 25 Apr 2014 00:50
Last Modified: 13 Oct 2017 03:18
URI: http://eprints.ums.edu.my/id/eprint/8849

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